Metagenomic Sequencing: Fast & Precise Pathogen & Microbiome Test
First of all, Metagenomic Sequencing (Shotgun Whole Genome Metagenomics) is the ultimate diagnostic technology that sequences all DNA present in a biological sample. Essentially, untargeted Next-Generation Sequencing (NGS) profiles bacteria, viruses, fungi, parasites, and antimicrobial resistance (AMR) genes simultaneously with 99.9% accuracy. As a result, clinicians identify mysterious pathogens and complex gut dysbiosis without relying on traditional cultures.
Book your Metagenomic Next-Generation Sequencing at Henotic Diagnostics for comprehensive diagnostic precision. Furthermore, our accredited diagnostic facility operates 24/7 with dedicated home sample collection services.
📋 Your Metagenomic Sequencing at a Glance
| 🔍 Detail | 📋 Info |
|---|---|
| 🩺 Technology | Shotgun Whole Genome Metagenomic NGS |
| 🩸 Sample Type | Stool / Plasma / CSF / Tissue / BAL Sample |
| 💰 Price Range | ₹35,000 – ₹55,000 (All-inclusive diagnostic fee) |
| 📄 Report TAT | 10 to 14 Days (Includes bioinformatic alignment) |
| 🕐 Availability | 24/7 Operations (Home sample collection available) |
| 👩⚕️ Specialist | Dr. Pratibha Patil — Chief Consultant Radiologist |
| 🍽️ Fasting | No Fasting Required (Normal diet maintained) |
| 💳 Insurance | Reimbursement Documentation Support provided |
| 🏅 Accreditation | NABL Accredited · ISO Certified · CAP Compliant |
Metagenomic Sequencing Quality Standards & Certifications
Overview of Metagenomic Sequencing
To begin with, Metagenomic Sequencing bypasses traditional culture isolation by sequencing total DNA extracted directly from clinical specimens. At the same time, experts at Henotic Diagnostics deploy shotgun NGS pipelines to analyze human, bacterial, viral, fungal, and parasitic genomes in parallel.
What Does Metagenomic Sequencing Evaluate?
Specifically, shotgun metagenomics sequences unamplified genomic DNA fragments to identify pathogenic species alongside functional metabolic genes and antibiotic resistance markers. Consequently, bioinformatic algorithms subtract human host DNA and map microbial reads against reference databases. Furthermore, high-depth sequencing detects culture-negative infectious agents and rare microdeletions. As a result, physicians solve refractory diagnostic dilemmas rapidly. In fact, to review foundational metagenomic research, consult the NIH Human Microbiome Project study.
Furthermore, comfortable reception lounge ensuring a smooth, stress-free sample submission experience
What is Metagenomic Sequencing?
In general, shotgun metagenomics randomly shears all DNA extracted from a clinical specimen into small fragments. Essentially, Next-Generation Sequencers generate gigabases of raw sequence reads representing all biological kingdoms present. Specifically, advanced software aligns reads against curated microbial databases to quantify organism abundance. Therefore, clinicians obtain a comprehensive diagnostic profile without choosing specific PCR targets in advance. Additionally, to read about technological advances, examine the Nature Biotechnology Metagenomics overview.
Additionally, high-throughput shotgun NGS platforms sequence unamplified total DNA with 99.9% accuracy
What Does Metagenomic Sequencing Check?
Overall, clinical shotgun metagenomics screens across multiple diagnostic categories:
Key Pathogen & Genomic Targets Evaluated
In addition, bioinformatic software maps multi-kingdom microbial trees and resistance gene profiles
When Is Metagenomic Sequencing Recommended?
For example, infectious disease specialists, gastroenterologists, and critical care physicians order Shotgun Whole Genome Metagenomics for severe, unexplained infections. In particular, testing is recommended for Pyrexia of Unknown Origin (PUO) or culture-negative sepsis. Moreover, it is advised for complex gut dysbiosis, SIBO, and inflammatory bowel disorders. In addition, to verify diagnostic validation standards, review the FDA Next-Generation Sequencing guidelines.
Moreover, expert clinical consultation translates shotgun metagenomic data into targeted antimicrobial plans
Clinical Superiority: Untargeted Metagenomics vs. Target-Specific PCR
Ideally, clinicians should order Metagenomic Sequencing if target-specific PCR panels fail to identify an infectious organism. In addition, targeted PCR tests search only for pre-specified microbes, whereas shotgun sequencing screens all known pathogens. Indeed, to examine pathogen surveillance protocols, consult the WHO Global Infectious Disease Surveillance resource.
Metagenomic Sequencing Cost Guide
| Clinical Metagenomic Service | Approximate Cost (₹) |
|---|---|
| Shotgun Metagenomic Stool Microbiome Panel | First, gut metagenomic sequencing costs ₹35,000 – ₹42,500. |
| Cell-Free Metagenomic Pathogen Panel (Plasma/CSF) | Second, clinical infectious metagenomics costs ₹42,500 – ₹55,000. |
| Home Sample Collection Kit Delivery | In addition, home phlebotomy collection is complimentary. |
| Clinical Bioinformatic Report Consultation | Finally, expert consultation fee is ₹1,000 – ₹1,500. |
Comparison: Shotgun Metagenomics vs. 16S Amplicon Sequencing
On one hand, 16S Amplicon sequencing evaluates exclusively bacterial 16S ribosomal RNA genes, whereas Metagenomic Sequencing analyzes unamplified total DNA across bacteria, viruses, fungi, and host genes. Consequently, shotgun metagenomics offers superior species-level resolution. In fact, review the PubMed Clinical Metagenomics Diagnostic review.
| Feature | Metagenomic Sequencing (Shotgun) | 16S Amplicon Sequencing |
|---|---|---|
| Biological Scope | First, multi-kingdom (Bacteria, Viruses, Fungi, Parasites). | However, strictly restricted to bacterial domain. |
| Taxonomic Resolution | Second, strain-level & species-level resolution. | In contrast, primarily genus-level resolution. |
| AMR Gene Detection | In addition, direct profiling of drug resistance genes. | Meanwhile, zero direct resistance gene detection. |
| Clinical Application | Finally, culture-negative sepsis & total microbiome. | Likewise, routine bacterial dysbiosis screening. |
Frequently Asked Questions
Is a Metagenomic Sequencing test painful? ▼
However, stool sample collection is completely non-invasive, while blood sample draw involves only a mild needle prick.
Do I need to fast before Metagenomic Sequencing? ▼
Because total DNA sequencing is independent of blood glucose, fasting is unnecessary.
What organisms are detected by Metagenomic Sequencing? ▼
Generally, it detects all bacteria, DNA viruses, fungi, parasites, and antimicrobial resistance (AMR) genes simultaneously.
How long until I receive diagnostic Metagenomic results? ▼
Fortunately, comprehensive bioinformatic metagenomic reports are delivered within 10 to 14 business days.
What is the cost of Metagenomic Sequencing? ▼
Overall, high-precision Metagenomic Sequencing ranges from ₹35,000 to ₹55,000 all-inclusive.
Quick Appointment Booking
Schedule your Metagenomic Sequencing with accredited genomic experts.
Call: 088793 27184 WhatsApp BookingTest Summary
Reporting Clinical Diagnostics Specialist
Dr. Pratibha Patil
Chief Consultant Radiologist & Clinical Diagnostics Specialist
MBBS, MD (Radiodiagnosis) · 15+ Years Clinical Experience
Furthermore, your Metagenomic Sequencing data is interpreted by accredited clinical pathologists and bioinformaticians. Consequently, comprehensive reports empower physicians with accurate pathogen and resistance profiles.
Our Accreditations & Quality Standards
Certified excellence ensuring shotgun sequencing depth, host DNA subtraction precision, and patient privacy.
What Our Patients Say
Real feedback from patients who completed Metagenomic Sequencing with us.
“After weeks of unexplained fever, Metagenomic Sequencing pinpointed a rare fungal infection that blood cultures missed. Furthermore, targeted treatment saved my life!”
– Ramesh V.
Verified Patient Review“The phlebotomy sample collection was seamless and highly professional. In addition, the bioinformatic report gave our specialist complete clarity.”
– Meena K.
Verified Patient Review“Outstanding diagnostic laboratory with NABL accreditation. Henotic Diagnostics provides true state-of-the-art metagenomic testing.”
– Dr. Alok T.
Verified Physician ReviewRelevant Diagnostic Metagenomic & Genomic Services
Corporate Booking Office & Location
If you require Metagenomic Sequencing for pathogen profiling or gut dysbiosis evaluation, do not delay. Call to schedule your sample collection.
Henotic Diagnostics
Second floor, Millennium Empire, Business Park, Plot No 47, D Mart Rd, Sector 15, Kharghar, Navi Mumbai, Panvel, Maharashtra 410210
Phone: 088793 27184 | Hours: Open 24 Hours