16s Rrna Sequencing: Fast & Precise Bacterial Microbiome Test
First of all, 16s Rrna Sequencing (16S Ribosomal RNA Gene Sequencing) is the gold standard Next-Generation Sequencing (NGS) technique for profiling bacterial communities. Essentially, amplifying hypervariable V3-V4 gene regions identifies gut bacterial species, taxonomic diversity, phylum ratios, and metabolic dysbiosis with 99.9% accuracy. As a result, clinicians treat IBS, SIBO, autoimmune inflammation, and metabolic disorders effectively.
Book your 16S Amplicon Sequencing at Henotic Diagnostics for comprehensive microbiome insight. Furthermore, our accredited diagnostic facility operates 24/7 with convenient home sample collection services.
📋 Your 16s Rrna Sequencing at a Glance
| 🔍 Detail | 📋 Info |
|---|---|
| 🩺 Technology | 16S rRNA V3-V4 Amplicon Next-Generation Sequencing |
| 🩸 Sample Type | Stool Sample in DNA Preservation Buffer |
| 💰 Price Range | ₹12,500 – ₹22,500 (All-inclusive diagnostic fee) |
| 📄 Report TAT | 10 to 14 Days (Includes bioinformatic alignment) |
| 🕐 Availability | 24/7 Operations (Home sample collection available) |
| 👩⚕️ Specialist | Dr. Pratibha Patil — Chief Consultant Radiologist |
| 🍽️ Fasting | No Fasting Required (Normal diet maintained) |
| 💳 Insurance | Reimbursement Documentation Support provided |
| 🏅 Accreditation | NABL Accredited · ISO Certified · CAP Compliant |
16s Rrna Sequencing Quality Standards & Certifications
Overview of 16s Rrna Sequencing
To begin with, 16S Amplicon Sequencing evaluates the ribosomal RNA gene present in all prokaryotic bacterial genomes. At the same time, experts at Henotic Diagnostics sequence stool DNA to map hundreds of bacterial genera inhabiting your gastrointestinal tract.
What Does 16s Rrna Sequencing Evaluate?
Specifically, 16S amplicon sequencing targets Nine hypervariable regions (V1-V9), with V3-V4 providing optimal taxonomic resolution for genus and species identification. Consequently, bioinformatic algorithms profile beneficial probiotic strains (Bifidobacterium, Lactobacillus, Akkermansia) alongside opportunistic pathogens (Clostridium, Enterobacter). Furthermore, high-depth NGS measures alpha and beta diversity metrics. As a result, gastroenterologists craft targeted dietary and probiotic therapies. In fact, to review international microbiome research standards, consult the NIH Human Microbiome Project resource.
Furthermore, comfortable reception lounge providing discreet intake for stool DNA collection kits
What is 16s Rrna Sequencing?
In general, the 16S rRNA gene contains highly conserved regions interspersed with nine hypervariable regions unique to bacterial species. Essentially, universal PCR primers bind conserved sequences to amplify variable regions. Specifically, Next-Generation Sequencers generate millions of sequence reads which are matched against curated databases like SILVA or Greengenes. Therefore, 16S sequencing provides an accurate bacterial census without needing live bacterial culture. Additionally, this test pinpoints silent bacterial dysbiosis. In fact, to review international microbiome research standards, consult the NIH Human Microbiome Project resource.
Additionally, high-throughput NGS sequencers amplify bacterial 16S V3-V4 hypervariable genes with 99.9% precision
What Does 16s Rrna Sequencing Check?
Overall, 16S rRNA gene profiling evaluates core bacterial parameters:
Key Bacterial Parameters Evaluated
In addition, bioinformatic software maps bacterial taxonomic trees and phylum abundance charts
When Is 16s Rrna Sequencing Recommended?
For example, gastroenterologists, functional medicine doctors, and clinical nutritionists recommend 16S Amplicon Sequencing for chronic digestive issues. In particular, testing is advised for patients experiencing persistent bloating, Irritable Bowel Syndrome (IBS), or Small Intestinal Bacterial Overgrowth (SIBO). Moreover, it is recommended following long-term antibiotic therapy to measure gut flora recovery. In addition, individuals dealing with autoimmune skin conditions or metabolic fatigue order 16S sequencing for targeted probiotic restoration. In fact, to review international microbiome research standards, consult the NIH Human Microbiome Project resource.
Moreover, expert consultation translates complex 16S bacterial data into personalized gut health plans
Clinical Distinction: 16S rRNA Amplicon vs. Stool Bacterial Culture
Ideally, patients should choose 16S Amplicon Sequencing if traditional stool culture tests return normal despite chronic symptoms. In addition, over 85% of gut bacteria are obligate anaerobes that cannot grow on petri dishes. Indeed, 16S DNA sequencing detects non-culturable bacteria with complete precision. In fact, to review international microbiome research standards, consult the NIH Human Microbiome Project resource.
16s Rrna Sequencing Cost Guide
| Diagnostic Metagenomic Service | Approximate Cost (₹) |
|---|---|
| 16S rRNA V3-V4 Bacterial Profiling Panel | First, standard 16S sequencing costs ₹12,500 – ₹16,500. |
| Comprehensive Gut Microbiome (16S + Fungal ITS) | Second, dual bacterial & fungal panel costs ₹18,500 – ₹22,500. |
| Home Sample Collection Kit Delivery | In addition, home collection service is complimentary. |
| Clinical Bioinformatic Report Consultation | Finally, expert consultation fee is ₹1,000 – ₹1,500. |
Comparison: 16S Amplicon Sequencing vs. Shotgun Metagenomics
On one hand, Shotgun Metagenomic sequencing reads all genomic DNA (bacterial, viral, fungal, human), whereas 16S Amplicon Sequencing specifically targets bacterial taxonomy at a lower cost. Consequently, 16S sequencing is the most cost-effective tool for routine bacterial dysbiosis screening.
| Feature | 16s Rrna Sequencing (Amplicon) | Shotgun Metagenomics (WMS) |
|---|---|---|
| Target Marker | First, 16S rRNA gene hypervariable V3-V4. | However, total unamplified genomic DNA. |
| Kingdom Scope | Second, exclusively bacterial taxonomic profiling. | In contrast, bacteria, viruses, fungi, & parasites. |
| Cost Affordability | In addition, highly economical (₹12,500 – ₹16,500). | Meanwhile, higher cost (₹35,000 – ₹55,000). |
| Clinical Utility | Finally, routine dysbiosis & F/B ratio evaluation. | Likewise, complex functional gene pathway analysis. |
Frequently Asked Questions
Is a 16s Rrna Sequencing test painful? ▼
However, routine stool sample collection is completely non-invasive and painless.
Do I need to fast before 16s Rrna Sequencing? ▼
Because bacterial DNA profiling is unaffected by fasting, normal diet is maintained.
What sample is required for 16s Rrna Sequencing? ▼
Generally, a small stool sample in a specialized DNA preservation buffer is required.
How long until I receive diagnostic 16s Rrna results? ▼
Fortunately, detailed bioinformatic microbiome reports are delivered within 10 to 14 business days.
What is the cost of 16s Rrna Sequencing? ▼
Overall, high-precision 16s Rrna Sequencing ranges from ₹12,500 to ₹22,500 all-inclusive.
Quick Appointment Booking
Schedule your 16s Rrna Sequencing with accredited genomic experts.
Call: 088793 27184 WhatsApp BookingTest Summary
Reporting Clinical Microbiome Specialist
Dr. Pratibha Patil
Chief Consultant Radiologist & Clinical Diagnostics Specialist
MBBS, MD (Radiodiagnosis) · 15+ Years Clinical Experience
Furthermore, your 16s Rrna Sequencing data is processed through accredited bioinformatic pipelines aligning reads against the SILVA database. Consequently, detailed reports empower gastroenterologists with actionable bacterial insights.
Our Accreditations & Quality Standards
Certified excellence ensuring amplicon sequencing depth, taxonomic alignment accuracy, and patient data privacy.
What Our Patients Say
Real feedback from patients who completed 16S rRNA microbiome profiling with us.
“After suffering from chronic bloating for 2 years, 16s Rrna Sequencing identified severe Akkermansia deficiency. Furthermore, targeted prebiotic therapy resolved my symptoms!”
– Vikram S.
Verified Patient Review“The stool collection kit arrived directly at home with simple instructions. In addition, the bioinformatic report was easy to discuss with my gastroenterologist.”
– Deepa R.
Verified Patient Review“Transparent pricing and NABL accredited accuracy. Henotic Diagnostics provides top-tier metagenomic testing.”
– Siddharth M.
Verified Patient ReviewRelevant Diagnostic Microbiome & Genomic Services
Corporate Booking Office & Location
If you require 16s Rrna Sequencing for bacterial microbiome profiling, do not delay. Call to schedule your sample collection.
Henotic Diagnostics
Second floor, Millennium Empire, Business Park, Plot No 47, D Mart Rd, Sector 15, Kharghar, Navi Mumbai, Panvel, Maharashtra 410210
Phone: 088793 27184 | Hours: Open 24 Hours