Next Generation Sequencing: Fast & Precise NGS Test
First of all, Next Generation Sequencing (NGS) represents the pinnacle of modern molecular diagnostics, reading millions of DNA sequences in parallel. Essentially, high-throughput NGS technology profiles single nucleotide variants (SNVs), copy number alterations (CNVs), and gene fusions with 100x to 500x coverage depth. As a result, oncologists and medical geneticists pinpoint actionable mutations for precision medicine and rare disease diagnosis.
Book your Next Generation Sequencing at Henotic Diagnostics for unmatched genomic precision. Furthermore, our accredited diagnostic facility operates 24/7 to provide seamless sample collection.
📋 Your Next Generation Sequencing at a Glance
| 🔍 Detail | 📋 Info |
|---|---|
| 🩺 Technology | 100x-500x Massively Parallel High-Throughput NGS |
| 🩸 Sample Type | Blood EDTA / FFPE Tumor Tissue / Bone Marrow |
| 💰 Price Range | ₹22,500 – ₹45,000 (All-inclusive diagnostic fee) |
| 📄 Report TAT | 14 to 21 Days (Includes ACMG variant classification) |
| 🕐 Availability | 24/7 Operations (Home sample collection available) |
| 👩⚕️ Specialist | Dr. Pratibha Patil — Chief Consultant Radiologist |
| 🍽️ Fasting | No Fasting Required (Normal diet allowed) |
| 💳 Insurance | Reimbursement Documentation Support provided |
| 🏅 Accreditation | NABL Accredited · ISO Certified · CAP Compliant |
Next Generation Sequencing Quality Standards & Certifications
Overview of Next Generation Sequencing (NGS)
To begin with, Next Generation Sequencing (NGS) is an ultra-high-throughput DNA sequencing methodology that has revolutionized clinical genetics and oncology. At the same time, experts at Henotic Diagnostics deploy state-of-the-art NGS platforms to analyze gene panels, exomes, and whole genomes.
What Does Next Generation Sequencing Test Evaluate?
Specifically, NGS evaluates single nucleotide variants (SNVs), small insertions and deletions (indels), copy number variations (CNVs), and gene rearrangement fusions. Consequently, medical geneticists identify disease-causing mutations across broad gene panels. Furthermore, high-depth sequencing detects low-frequency somatic mutations in cancer tissue. As a result, oncologists select targeted therapies with high precision. In fact, to review clinical research standards, consult the NIH Clinical Next Generation Sequencing resource.
Furthermore, comfortable reception lounge ensuring a smooth, welcoming intake for genomic sample registration
What is Next Generation Sequencing?
In general, NGS is a massively parallel sequencing technology that reads millions of short DNA fragments simultaneously. Essentially, unlike traditional Sanger sequencing which processes one DNA strand at a time, NGS chemistry reads entire gene networks in a single instrument run. Specifically, fluorescently labeled nucleotides emit distinct optical signals as DNA polymerase synthesizes complementary strands. Therefore, bioinformatic algorithms align billions of reads against human reference databases to report ACMG-classified variants. Additionally, NGS underpins Whole Exome (WES), Whole Genome (WGS), and NIPT testing.
Additionally, high-throughput NGS sequencers process millions of DNA strands simultaneously with extreme accuracy
What Does Next Generation Sequencing Check?
Overall, NGS provides comprehensive variant detection across diverse clinical medical panels:
Major Clinical Next Generation Sequencing Applications
In addition, bioinformatic software pipelines filter sequence variants against global ClinVar databases
When Is Next Generation Sequencing Test Recommended?
For example, oncologists, neurologists, and clinical geneticists order Next Generation Sequencing for precise clinical indications. In particular, NGS is ordered for cancer patients to identify targetable biomarker mutations for immunotherapy or tyrosine kinase inhibitors. Moreover, it is recommended for pediatric patients presenting with unexplained developmental delays, dysmorphic features, or metabolic crises. In addition, individuals with a strong family history of early-onset hereditary conditions undergo NGS gene panel screening.
Moreover, expert genetic counseling translates complex NGS variant classifications into clear patient treatment plans
Clinical Advantage: NGS vs. Single-Gene Sanger Testing
Ideally, clinicians choose NGS if multiple candidate genes could explain a patient’s symptoms. In addition, if single-gene Sanger tests are run sequentially, diagnostic costs escalate while wasting precious time. Indeed, NGS tests dozens or hundreds of candidate genes simultaneously in one cost-effective workflow.
Next Generation Sequencing Cost Guide
| NGS Genomic Service | Approximate Cost (₹) |
|---|---|
| Targeted NGS Gene Panel (50-100 Genes) | First, targeted NGS panel pricing is ₹22,500 – ₹28,500. |
| Comprehensive Oncology NGS Panel (Solid Tumor) | Second, oncology NGS panel costs ₹32,500 – ₹45,000. |
| Whole Exome Sequencing NGS (Child / Proband) | In addition, Whole Exome NGS costs ₹18,000 – ₹25,000. |
| Pre & Post-Test Genetic Counseling | Finally, expert genetic consultation fee is ₹1,500 – ₹2,500. |
Comparison: Next Generation Sequencing vs. Sanger Sequencing
On one hand, Sanger sequencing processes a single DNA fragment per reaction, whereas Next Generation Sequencing reads millions of DNA fragments in parallel. Consequently, NGS is dramatically faster and cheaper for multi-gene disease panels.
| Feature | Next Generation Sequencing (NGS) | Sanger Sequencing |
|---|---|---|
| Throughput Capacity | First, sequences millions of DNA fragments concurrently. | However, sequences only 1 target DNA strand at a time. |
| Multi-Gene Panel Capability | Second, tests 50 to 20,000 genes in a single test run. | In contrast, multi-gene testing is extremely laborious & costly. |
| Somatic Mutation Sensitivity | In addition, high-depth NGS detects <1% low-frequency variants. | Meanwhile, Sanger requires at least 15-20% mutant allele frequency. |
| Primary Clinical Use | Finally, ideal for complex genetic panels, exomes, & oncology. | Likewise, ideal for single-gene familial mutation validation. |
Frequently Asked Questions
Is a Next Generation Sequencing test painful? ▼
However, routine peripheral blood collection involves only a standard needle prick, while genomic sequencing is laboratory-based.
Do I need to fast before Next Generation Sequencing? ▼
Because genomic DNA extraction relies on cellular DNA rather than blood sugar, fasting is unnecessary.
How long does the sample collection take? ▼
Generally, blood collection takes 5 minutes, while high-throughput sequencing and bioinformatic analysis requires 14 to 21 days.
How long until I receive diagnostic results? ▼
Fortunately, detailed ACMG-classified variant reports are delivered within 14 to 21 business days.
What is the cost of Next Generation Sequencing? ▼
Overall, a high-precision Next Generation Sequencing test ranges from ₹22,500 to ₹45,000 all-inclusive.
Quick Appointment Booking
Schedule your Next Generation Sequencing with accredited genomic experts.
Call: 088793 27184 WhatsApp BookingTest Summary
Reporting Clinical Diagnostic Specialist
Dr. Pratibha Patil
Chief Consultant Radiologist & Clinical Diagnostics Specialist
MBBS, MD (Radiodiagnosis) · 15+ Years Clinical Experience
Furthermore, your Next Generation Sequencing data is evaluated and reported by senior clinical bioinformaticians and molecular pathologists following ACMG guidelines. Consequently, detailed reports provide treating physicians with definitive evidence for patient care.
Our Accreditations & Quality Standards
Certified excellence ensuring NGS sequencing depth, variant calling precision, and patient privacy.
What Our Patients Say
Real feedback from patients who completed Next Generation Sequencing with us.
“Targeted NGS gene testing at Henotic Diagnostics identified the exact EGFR mutation in my father’s lung biopsy. Furthermore, the targeted drug treatment has been remarkably effective.”
– Arvind S.
Verified Patient Review“Seamless blood sample collection at home for our hereditary cancer panel. In addition, the genetic counselor explained the ACMG report with immense clarity.”
– Rekha V.
Verified Patient Review“Transparent price and NABL accredited accuracy. Dr. Pratibha Patil’s lab provides world-class molecular sequencing.”
– Dr. Jayant K.
Verified Patient ReviewRelevant Diagnostic Genomic & Molecular Services
Corporate Booking Office & Location
If you require Next Generation Sequencing for molecular diagnostic or genetic evaluation, do not delay. Call to schedule your sample collection.
Henotic Diagnostics
Second floor, Millennium Empire, Business Park, Plot No 47, D Mart Rd, Sector 15, Kharghar, Navi Mumbai, Panvel, Maharashtra 410210
Phone: 088793 27184 | Hours: Open 24 Hours